anti aβ rabbit polyclonal primary antibody  (Bioss)

Journal: Epigenetics & Chromatin

Article Title: Interaction of serotonin transporter and TGFB1 facilitates histone serotonylation-mediated synaptic plasticity following environmental enrichment in stressed mouse Mus booduga

doi: 10.1186/s13072-025-00653-y

Figure Lengend Snippet: Environmental enrichment (EE) confers resilience to social isolation-induced alterations in the expression of serotonin transporter (SERT) and transforming growth factor-B1 (TGFB1), and increases their interaction. Western blotting images show the expression levels of SERT, TGFB1, and SERT + TGFB1 (co-immunoprecipitation). ( a ) Estimated values show that EE reverses SI stress-induced changes in the expression of ( b ) TGFB1, ( c ) SERT, and ( d ) interaction of TGFB1 with SERT. Data are presented as mean ± SD [Direct from Wild (DW; n = 6), Short-Term at Standard Condition (STSC; n = 6), Long-Term at Standard Condition (LTSC; n = 6), Environmental Enrichment (EE; n = 6)]. Kruskal-Wallis test followed by Student-Newman-Keuls pairwise comparison test: * indicates level of significance (* p < 0.05)

Article Snippet: An equal volume of anti-SERT antibody-precipitated protein was processed for western blot analysis (as mentioned earlier) using primary antibody anti- TGFB1 rabbit polyclonal antibody (Cat # 3711, 1:1000; Cell Signaling Technology) and secondary antibody goat anti-rabbit IgG-ALP (Cat# 621100180011730; 1:5000; GeNeiTM).

Techniques: Isolation, Expressing, Western Blot, Immunoprecipitation, Comparison

Journal: Epigenetics & Chromatin

Article Title: Interaction of serotonin transporter and TGFB1 facilitates histone serotonylation-mediated synaptic plasticity following environmental enrichment in stressed mouse Mus booduga

doi: 10.1186/s13072-025-00653-y

Figure Lengend Snippet: Environmental enrichment (EE) possibly increases the interaction between serotonin transporter (SERT) and transforming growth factor-B1 (TGFB1) complex. Two-dimensional western blot images showing the presence of SERT at 60 kDa with pI 5.87 (immaturely-glycosylated SERT) and 152 kDa with pI 7.83 (mature SERT + TGFB1 complex). Functional SERT was observed in DW ( a ) and EE ( d ) mice, and immaturely glycosylated SERT in stressed mice under STSC ( b ) and EE ( d ) mice. [Direct from Wild (DW), Short-Term at Standard Condition (STSC), Long-Term at Standard Condition (LTSC), Environmental Enrichment (EE)]

Article Snippet: An equal volume of anti-SERT antibody-precipitated protein was processed for western blot analysis (as mentioned earlier) using primary antibody anti- TGFB1 rabbit polyclonal antibody (Cat # 3711, 1:1000; Cell Signaling Technology) and secondary antibody goat anti-rabbit IgG-ALP (Cat# 621100180011730; 1:5000; GeNeiTM).

Techniques: Western Blot, Functional Assay

Journal: Epigenetics & Chromatin

Article Title: Interaction of serotonin transporter and TGFB1 facilitates histone serotonylation-mediated synaptic plasticity following environmental enrichment in stressed mouse Mus booduga

doi: 10.1186/s13072-025-00653-y

Figure Lengend Snippet: Environmental enrichment (EE) possibly increases the interaction between serotonin transporter (SERT) and transforming growth factor-B1 (TGFB1) complex. Two-dimensional western blot image showing the presence of TGFB1 at 44 kDa with a pI of 8.30 and 152 kDa with a pI of 7.83 (SERT + TGFB1 complex). SERT + TGFB1 complex was observed in DW ( a ) and EE ( d ) mice, and TGFB1 was observed in all experimental groups [Direct from Wild (DW), Short-Term at Standard Condition (STSC), Long-Term at Standard Condition (LTSC), Environmental Enrichment (EE)]

Article Snippet: An equal volume of anti-SERT antibody-precipitated protein was processed for western blot analysis (as mentioned earlier) using primary antibody anti- TGFB1 rabbit polyclonal antibody (Cat # 3711, 1:1000; Cell Signaling Technology) and secondary antibody goat anti-rabbit IgG-ALP (Cat# 621100180011730; 1:5000; GeNeiTM).

Techniques: Western Blot

Journal: Veterinary Sciences

Article Title: PDGFRβ Expression Across Canine AGASAC Subtypes and Metastases: Morphologic Insights and Possible Therapeutic Implications †

doi: 10.3390/vetsci12121122

Figure Lengend Snippet: Microscopic images of canine AGASAC growth patterns. ( A , B ) Pure solid pattern: ( A ) Lobules of neoplastic cells with scant stroma, surrounded by desmoplasia; ( B ) PDGFRβ is variably expressed in neoplastic cells’ cytoplasm and membrane. Blood vessel endothelial cells and stromal CAFs are PDGFRβ-positive. ( C , D ) Pure tubular pattern: ( C ) Neoplastic cells in well-formed tubules; ( D ) PDGFRβ is strongly expressed in the tubular epithelium’s cytoplasm and endothelial cells. ( E , F ) Mixed pattern: ( E ) Areas with tubular and solid growth; ( F ) PDGFRβ expression is heterogeneous, with strong labeling in tubules and weaker in solid areas. CAFs are visible in the stroma. ( G , H ) Comedo-carcinoma pattern: ( G ) Solid sheets with necrosis; ( H ) neoplastic cells and CAFs have moderate cytoplasmic PDGFRβ. ( I , J ) Neuroendocrine-like pattern (rosettes and packets): ( I ) Small, scattered to confluent packets; ( J ) PDGFRβ varies, often labeling scattered cells from strong to weak. ( A , C , E , G , I )—H&E stain; ( B , D , F , H , J )—PDGFRβ expression, evaluated by immunohistochemistry with DAB chromogen.

Article Snippet: PDGFRβ expression was evaluated on 4 μm paraffin sections of the primary AGASAC and metastases, mounted on poly-L-lysine-coated slides [ , ], and stained with rabbit polyclonal anti-human PDGFRβ primary antibody (3162S, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Membrane, Expressing, Labeling, Staining, Immunohistochemistry

Journal: Veterinary Sciences

Article Title: PDGFRβ Expression Across Canine AGASAC Subtypes and Metastases: Morphologic Insights and Possible Therapeutic Implications †

doi: 10.3390/vetsci12121122

Figure Lengend Snippet: PDGFRβ immunohistochemical expression in primary AGASACs and corresponding metastasis. ( A ) Primary AGASAC with tubular growth pattern demonstrating diffuse, intense, cytoplasmic PDGFRβ expression. ( B ) Corresponding metastatic lesion in the sacral lymph node, characterized by a shift toward a more solid growth pattern and reduced, faint PDGFRβ staining.

Article Snippet: PDGFRβ expression was evaluated on 4 μm paraffin sections of the primary AGASAC and metastases, mounted on poly-L-lysine-coated slides [ , ], and stained with rabbit polyclonal anti-human PDGFRβ primary antibody (3162S, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Immunohistochemical staining, Expressing, Staining